کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2016529 | 1067667 | 2010 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and characterization of an acyl-CoA:diacylglycerol acyltransferase 2 (DGAT2) gene from the microalga O. tauri
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کلمات کلیدی
PAPVLCPUFADGTAPDATMGATLPAATGPATDGAT2ACATsn-1,2-diacylglycerol - sn-1،2-دی سیل گلیسرولOstreococcus tauri - استروکوکوس تاوریphosphatidic acid - اسید فسفاتیدیکFatty acids - اسیدهای چربVery long-chain polyunsaturated fatty acids - اسیدهای چرب غیر اشباع بسیار طولانیTriacylglycerols - ترشیال گلیسیرینtriacylglycerol - تری آسیل گلیسرول TAG یا triacylglycerols - تری گلیسرید یا تری آسیل گلیسرولDAG - روزMicroalgae - ریز جلبک هاendoplasmic reticulum - شبکه آندوپلاسمی phosphatidylcholine - فسفاتیدیل کولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification and characterization of an acyl-CoA:diacylglycerol acyltransferase 2 (DGAT2) gene from the microalga O. tauri Identification and characterization of an acyl-CoA:diacylglycerol acyltransferase 2 (DGAT2) gene from the microalga O. tauri](/preview/png/2016529.png)
چکیده انگلیسی
In order to identify novel genes encoding enzymes involved in the terminal step of triacylglycerol (TAG) formation, a database search was carried out in the genome of the unicellular photoautotrophic green alga Ostreococcus tauri. The search led to the identification of three putative type 2 acyl-CoA:diacylglycerol acyltransferase-like sequences (DGAT; EC 2.3.1.20), and revealed the absence of any homolog to type 1 or type 3 DGAT sequence in the genome of O. tauri. For two of the cDNA sequences (OtDGAT2A and B) enzyme activity was detected by heterologous expression in Saccharomyces cerevisiae mutant strains with impaired TAG metabolism. However, activity of OtDGAT2A was too low for further analysis. Analysis of their amino acid sequences showed that they share limited identity with other DGAT2 from different plant species, such as Ricinus communis and Vernicia fordii with â¼25 to 30% identity. Lipid analysis of the mutant yeast cells revealed that OtDGAT2B showed broad substrate specificity accepting saturated as well as mono- and poly-unsaturated acyl-CoAs as substrates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 48, Issue 6, June 2010, Pages 407-416
Journal: Plant Physiology and Biochemistry - Volume 48, Issue 6, June 2010, Pages 407-416
نویسندگان
Martin Wagner, Katharina Hoppe, Tibor Czabany, Mareike Heilmann, Günther Daum, Ivo Feussner, Martin Fulda,