کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2088832 | 1080816 | 2009 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial DNA replication and mtDNA-encoded protein synthesis
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کلمات کلیدی
NRTIPBSd4TmAbHEPESDDImAbs4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid - 4- (2-Hydroxyethyl) piperazine-1-ethanesulfonic acidddC - DDCMitochondrial DNA - DNA میتوکندریاMonoclonal antibody - آنتی بادی مونوکلونالAntibacterials - آنتی باکتریال هاSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدSodium dodecyl sulfate polyacrylamide gel electrophoresis - الکتروفورز ژل پلی اتیل آمید سدیم دودسیل سولفاتstandard deviation - انحراف معیارLateral-flow immunoassay - ایمونویسی امواج جانبیPopulation doubling - جمعیت دو برابر می شودmtDNA - دیانای میتوکندریاییToxicity - سمی بودنAntiretrovirals - ضد رترو ویروسیPhosphate buffered saline - فسفات بافر شورnucleoside reverse transcriptase inhibitor - مهار کننده ترانس کپیکول معکوس نوکلئوزیدNucleoside reverse transcriptase inhibitors - مهار کننده های ترانس کپسول معکوس نوکلئوزیدMitochondria - میتوکندریاAntibody - پادتَن یا آنتیبادی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial DNA replication and mtDNA-encoded protein synthesis Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial DNA replication and mtDNA-encoded protein synthesis](/preview/png/2088832.png)
چکیده انگلیسی
Drug-induced mitochondrial toxicity can occur as a result of inhibition of mitochondrial DNA (mtDNA) replication as with certain nucleoside reverse transcriptase inhibitors or inhibition of mtDNA-encoded protein synthesis as with certain antibacterials. Both types of dysfunction have the overall effect of reducing the level of proteins encoded by mtDNA. A lateral-flow immunoassay which measures the levels of both a mtDNA-encoded protein and a nuclear DNA-encoded protein allows simple and rapid determination of the ratio of these 2 proteins and, hence, identifies changes in mtDNA-encoded protein levels. Here, we describe an assay that compares the level of Complex IV (cytochrome c oxidase), a mitochondrial protein which has 3 subunits encoded by mtDNA and made by mitochondrial ribosomes, with that of frataxin, a protein encoded by nuclear DNA and made by cytosolic ribosomes. We tested a selection of antibacterials and antiretrovirals in cells and show that the ratio of Complex IV: frataxin decreases when a drug inhibits either mtDNA replication or mtDNA-encoded protein synthesis. The results obtained with the assay were confirmed by Western blotting and immunocytochemical analysis. The assay has high reproducibility, requires small amounts of sample, is quantitative, and is able to identify drugs which ultimately lead to a decrease in mtDNA-encoded proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 343, Issue 1, 31 March 2009, Pages 1-12
Journal: Journal of Immunological Methods - Volume 343, Issue 1, 31 March 2009, Pages 1-12
نویسندگان
Sashi Nadanaciva, John H. Willis, Melissa L. Barker, Dima Gharaibeh, Roderick A. Capaldi, Michael F. Marusich, Yvonne Will,