کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2130274 | 1086548 | 2014 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Dynamics of apelin receptor/G protein coupling in living cells Dynamics of apelin receptor/G protein coupling in living cells](/preview/png/2130274.png)
• We analyze the dynamics of G protein coupling to apelin receptor in living cells upon activation.
• It revealed that Gαi2, Gαi3, Gαo, and Gαq were involved in APJ activation.
• Gαi2, Gαi3 activation upon apelin-13 stimulation undergo a rearrangement instead of classic disassociation with Gβγ.
During our research on apelin receptor (APJ) signalling in living cells with BRET and FRET, we demonstrated that apelin-13 stimulation can lead to the activation of Gαi2 or Gαi3 through undergoing a molecular rearrangement rather than dissociation in HEK293 cells expressing APJ. Furthermore, Gαo and Gαq also showed involvement in APJ activation through a classical dissociation model. However, both FRET signal and BRET ratio between fluorescent Gαi1 subunit and Gβγ subunits demonstrated little change after apelin-13 stimulation. These results demonstrated that stimulation of APJ with apelin-13 causes activation of Gαi2, Gαi3, Gαo, Gαq; among which Gαi2, Gαi3 were activated through a novel rearrangement process. These results provide helpful data for understanding APJ mediated G-protein signalling.
Figure optionsDownload high-quality image (113 K)Download as PowerPoint slide
Journal: Experimental Cell Research - Volume 328, Issue 2, 1 November 2014, Pages 401–409