کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
225058 464473 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pulsed electric field in combination with vacuum impregnation with trehalose improves the freezing tolerance of spinach leaves
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Pulsed electric field in combination with vacuum impregnation with trehalose improves the freezing tolerance of spinach leaves
چکیده انگلیسی

Pulsed electric fields in combination with vacuum infusion have been utilized to impregnate cells with trehalose, aiming at substantially improving the freezing tolerance of spinach leaves. Spinach samples were first treated with ten trains of bi-polar, rectangular electric field pulses with a nominal electric field strength of 580 V/cm and immediately immersed in a 40% (w/w) solution of trehalose under vacuum for 20 min. The samples were kept in the trehalose solution for 2.5 h at atmospheric pressure, immersed in deionised water at 4 °C overnight, frozen in liquid nitrogen and thawed in water at room temperature. The leaves were evaluated for cell damage with microscopic observations and wilting tests. The results provided evidence that the impregnation with trehalose by the combined actions of electric fields and vacuum impregnation drastically improved the freezing tolerance of the spinach leaves.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Food Engineering - Volume 88, Issue 1, September 2008, Pages 144–148
نویسندگان
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