کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
226230 | 464530 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Hydrolysis of inulin by immobilized thermostable extracellular exoinulinase from Aspergillus fumigatus Hydrolysis of inulin by immobilized thermostable extracellular exoinulinase from Aspergillus fumigatus](/preview/png/226230.png)
The extracellular thermostable inulinase from Aspergillus fumigatus, which converts inulin into fructose, was immobilized on different supports after partial purification by ammonium sulphate precipitation, and ion exchange chromatography on DEAE-Sephacel. The optimum pH for free and the enzyme immobilized on various supports, was 5.5, whereas temperature optimum was 60 °C. The Km values of various immobilized inulinases, with inulin, varied from 0.2 to 0.5 mM. The heat stability of the enzyme was markedly improved by immobilization on various supports and the immobilized enzyme retained approximately 70% activity after 48 h at 60 °C. The operational half- life of the immobilized inulinase at 60 °C with 2.5% inulin was 22, 35 and 45 days for QAE-Sephadex, chitin and ConA packed bed column reactors, respectively, which makes this enzyme, in the immobilized form, a potential candidate for production of fructose syrups on industrial scale.
Journal: Journal of Food Engineering - Volume 76, Issue 3, October 2006, Pages 369–375