کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
23850 43478 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of rare codon clusters on the expression of a high-turnover chloroplast protein in Chlamydomonas reinhardtii
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Effects of rare codon clusters on the expression of a high-turnover chloroplast protein in Chlamydomonas reinhardtii
چکیده انگلیسی

Expression of foreign proteins in chloroplasts has become an important field of plant genetic engineering. Optimized codon usage is generally thought to increase translational efficiency, but high speed translation of codon bias-adjusted mRNAs can also result in protein misfolding due to a lack of rare codons. In order to analyze the effect of rare codons on a native chloroplast protein in vivo, we modified the D1 subunit of photosystem II by fusing small peptides with different codons into a loop region which tolerates insertions without loss of function. Because of its high-turnover properties, the D1 protein represents an excellent test object to investigate the impact of rare codons on its translation. We choose codons for amino acids Arg, Leu, Ser, Ala and Gly which are rarely used and compared translation of the modified D1 proteins with the respective mutant proteins containing insertions with frequently used codons. Our data indicate that only rare Arg codons drastically affect synthesis of the D1 protein and cluster of rare Ser-codon can induce strategic ribosomal pausing sites.


► Expression of foreign proteins in chloroplasts needs codon adjustment.
► We established a codon test system involving a native protein with a high turnover.
► Codon are identified which allow poor expression and/or translational pausing.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 160, Issues 3–4, 31 August 2012, Pages 105–111
نویسندگان
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