کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2405817 1103050 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A DNA vaccine targeting the receptor-binding domain of Clostridium difficile toxin A
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
A DNA vaccine targeting the receptor-binding domain of Clostridium difficile toxin A
چکیده انگلیسی

Clostridium difficile is a pathogen with increasing severity for which host antibody responses provide protection from disease. DNA vaccination has several advantages compared to traditional vaccine methods, however no study has examined this platform against C. difficile toxins. A synthetic gene was created encoding the receptor-binding domain (RBD) of C. difficile toxin A, optimized for expression in human cells. Gene expression was examined in vitro. Mice were inoculated and then challenged with parenteral toxin A. Vaccination provided high titer antibodies and protected mice from death. This represents the first report of DNA vaccine inducing neutralizing antibodies to C. difficile toxin A.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 27, Issue 27, 2 June 2009, Pages 3598–3604
نویسندگان
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