کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2407314 1103121 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The degree of apoptosis as an immunostimulant for a DNA vaccine against HIV-1 infection
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
The degree of apoptosis as an immunostimulant for a DNA vaccine against HIV-1 infection
چکیده انگلیسی

To regulate the expression of the apoptotic gene, we constructed bicistronic DNA vaccines that encode for HIV env and caspase-3 mutant (casp 3m) that are expressed via the encephalomyocarditis virus internal ribosomal entry site (IRES) or cytomegalovirus (CMV) promoter-dependent translations. While IRES-casp 3m induced weak apoptosis and caused little reduction in antigen expression, CMV-casp 3m elicited strong apoptosis and led to a marked decrease in the antigen expression. Therefore, IRES-casp 3m augmented HIV-specific immune responses, and IRES-casp 3m induced significant protection against the vaccinia-HIV chimeric virus. These results suggest that the appropriate level of apoptosis is important for DNA vaccine development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 25, Issue 3, 5 January 2007, Pages 438–445
نویسندگان
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