کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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24141 | 43500 | 2010 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Cross-linking and immobilisation of different proteins with recombinant Verrucomicrobium spinosum tyrosinase Cross-linking and immobilisation of different proteins with recombinant Verrucomicrobium spinosum tyrosinase](/preview/png/24141.png)
This paper reports on the cross-linking and immobilisation of various proteins by the recombinant tyrosinase from Verrucomicrobium spinosum (Vs-tyrosinase). In general it is found that Vs-tyrosinase can readily cross-link proteins with a low degree of complexity, such as casein, but that the enzyme cannot readily cross-link well folded protein substrates such as lysozyme, myoglobin, cytochrome c or Candida antarctica lipase B (CALB). However, the inclusion of phenolic compounds (phenol or caffeic acid) to reaction mixtures of these proteins can greatly enhance the levels of cross-linking. For example it is possible to prepare cross-linked aggregates of industrially applicable enzymes such as CALB by simply incubating it with Vs-tyrosinase and phenol. The resulting aggregates can be collected by centrifugation and retain high levels of activity and may find applications in biocatalysis.
Journal: Journal of Biotechnology - Volume 150, Issue 4, December 2010, Pages 546–551