کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2484486 | 1114313 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of Novel Insulin Fibrils That Show Strong Cytotoxicity Under Physiological pH
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کلمات کلیدی
ATR-FTIRDMSO - DMSOTem - این استThT - بلهprotein aggregation - تجمع پروتئینThioflavin T - تیوفلاوین TStability - ثباتSelf-assembly - خودآموزیDimethyl sulfoxide - دیمتیل سولفواکسیدProtein structure - ساختار پروتئینToxicity - سمی بودنFTIR - طیف سنج مادون قرمزCircular dichroism spectroscopy - طیف سنجی دی کرومattenuated total reflectance Fourier transform infrared spectroscopy - طیف سنجی مادون قرمز تبدیل فوریه تضعیف بازتاب کاملProtein formulation - فرمول پروتئینMALDI-TOF MS - مالدی توف MSMorphology - مورفولوژی(ریخت شناسی)Transmission electron microscopy - میکروسکوپ الکترونی عبوریProtein - پروتئینPeptide - پپتید
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Amyloid fibrils are β-sheet-rich protein aggregates that are associated with more than 20 diseases. Insulin is known to form amyloid fibrils under a variety of conditions in vitro. Insulin fibrillations have been generally performed under acidic conditions, which are conducive to the formation of fibrils. As insulin is found almost exclusively as a monomer in acidic solutions, insulin fibrillation under acidic conditions is proposed to occur via its monomer. However, insulin fibrils, which cause injection-localized amyloidosis, form under neutral pH conditions in vivo, because both subcutaneous tissue and almost all insulin formulations maintain a neutral pH. In this study, we induced fibrillation under conditions more closely resembling physiological conditions than those used in previous studies with the aim of better understanding the nature of injection-localized amyloidosis in vivo. The results of transmission electron microscopy, structural analyses, and MTT assay show that the fibrils formed under conditions more closely resembling physiological conditions have different properties from the fibrils described to date. The results of this study indicate that fibrils formed under conditions more closely resembling physiological conditions have different properties from insulin fibrils induced under the conditions reported in previous studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 105, Issue 4, April 2016, Pages 1419-1426
Journal: Journal of Pharmaceutical Sciences - Volume 105, Issue 4, April 2016, Pages 1419-1426
نویسندگان
Hiromu Yoshihara, Jun Saito, Ayaka Tanabe, Takuma Amada, Toshinari Asakura, Kouki Kitagawa, Shinichi Asada,