کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2486137 | 1556514 | 2011 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biophysical Characterization and Conformational Stability of Ebola and Marburg Virus-Like Particles
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Biophysical Characterization and Conformational Stability of Ebola and Marburg Virus-Like Particles Biophysical Characterization and Conformational Stability of Ebola and Marburg Virus-Like Particles](/preview/png/2486137.png)
چکیده انگلیسی
The filoviruses, Ebola virus and Marburg virus, cause severe hemorrhagic fever with up to 90% human mortality. Virusâlike particles of EBOV (eVLPs) and MARV (mVLPs) are attractive vaccine candidates. For the development of stable vaccines, the conformational stability of these two enveloped VLPs produced in insect cells was characterized by various spectroscopic techniques over a wide pH and temperature range. Temperatureâinduced aggregation of the VLPs at various pH values was monitored by light scattering. Temperature/pH empirical phase diagrams (EPDs) of the two VLPs were constructed to summarize the large volume of data generated. The EPDs show that both VLPs lose their conformational integrity above about 50°C-60°C, depending on solution pH. The VLPs were maximally thermal stable in solution at pH 7-8, with a significant reduction in stability at pH 5 and 6. They were much less stable in solution at pH 3-4 due to increased susceptibility of the VLPs to aggregation. The characterization data and conformational stability profiles from these studies provide a basis for selection of optimized solution conditions for further vaccine formulation and longâterm stability studies of eVLPs and mVLPs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 100, Issue 12, December 2011, Pages 5156-5173
Journal: Journal of Pharmaceutical Sciences - Volume 100, Issue 12, December 2011, Pages 5156-5173
نویسندگان
Lei Hu, Jared M. Trefethen, Yuhong Zeng, Luisa Yee, Satoshi Ohtake, David LechugaâBallesteros, Kelly L. Warfield, M. Javad Aman, Sergey Shulenin, Robert Unfer, Sven G. Enterlein, Vu TruongâLe, David B. Volkin, Sangeeta B. Joshi, C. Russell Middaugh,