کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2502366 1557378 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production, purification and biological characterization of mono-PEGylated anti-IL-17A antibody fragments
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی علوم دارویی
پیش نمایش صفحه اول مقاله
Production, purification and biological characterization of mono-PEGylated anti-IL-17A antibody fragments
چکیده انگلیسی

The aim of this study was to maximize the yield of the production of mono-PEGylated anti-interleukin-17A (anti-IL-17A) antibody fragments using large (≥20 kDa) polyethylene glycol (PEG) chains. Particular attention was paid to selectively yield mono-PEGylated species to maintain the maximum possible functionality and to simplify the purification. Neutralization of IL-17A by antibody constructs might find application for the treatment of bronchial hyperreactivity. Amino-directed and sulfhydryl-directed PEGylation of the native antibody fragments were compared. The former was selected as it produced the most interesting construct in terms of yield and preservation of biological activity. In particular, the F(ab′)2-PEG conjugate with one 40 kDa branched PEG prepared in this study was produced at a 42% yield. The conjugate presented only a slight decrease in its binding activity and in its in vitro inhibitory potency offering interesting perspectives for in vivo studies.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Pharmaceutics - Volume 454, Issue 1, 15 September 2013, Pages 107–115
نویسندگان
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