کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2507838 | 1117511 | 2014 | 5 صفحه PDF | دانلود رایگان |

A simple, fast and sensitive capillary electrophoresis (CE) strategy combined with chemiluminescence (CL) detection for analysis of ofloxacin (OF) enantiomers was established in the present work. Sulfonated β-cyclodextrin (β-CD) was used as the chiral additive being added into the running buffer of luminol–diperiodatocuprate (III) (K5[Cu(HIO6)2], DPC) chemiluminescence system. Under the optimum conditions, the proposed method was successfully applied to separation and analysis of OF enantiomers with the detection limits (S/N=3) of 8.0 nM and 7.0 nM for levofloxacin and dextrofloxacin, respectively. The linear ranges were both 0.010–100 μM. The method was utilized for analyzing OF in urine; the results obtained were satisfactory and recoveries were 89.5–110.8%, which demonstrated the reliability of this method. This approach can also be further extended to analyze different commercial OF medicines.
Journal: Journal of Pharmaceutical Analysis - Volume 4, Issue 6, December 2014, Pages 412–416