کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2507977 | 1117521 | 2013 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Determination and stress studies on YK-1101, a potential histone deacetylase, by HPLC–UV and HPLC–TOF/MS methods Determination and stress studies on YK-1101, a potential histone deacetylase, by HPLC–UV and HPLC–TOF/MS methods](/preview/png/2507977.png)
YK-1101, with its structure as S-((E)-4-((7S,10S,Z)-4-ethylidene-7-isopropyl-2,5,8,12-tetraoxo -9-oxa-16-thia-3,6,13,18-tetraazabicyclo[13.2.1]octadeca-1(17),15(18)-dien-10-yl)but-3-en-1-yl) ethanethioate, is synthesized as a potential histone deacetylase inhibitor. Its quality and stability under various stress conditions are not fully understood. In this study, a high performance liquid chromatographic (HPLC) method was established and validated for the analysis of YK-1101 bulk drug samples. The chromatographic separation was performed on a C18 column with acetonitrile and water as mobile phase in a gradient elution. Based on the established method, the stability studies of YK-1101 under various stress conditions were carried out. YK-1101 was shown to undergo degradation under basic and acidic stress conditions, while it was stable under oxidative, photolytic and thermal conditions. In addition, a time of flight mass spectrometer (TOF/MS) was coupled to HPLC for the characterization of major degradation products produced under basic and acidic stress conditions. Their degradation pathways were also discussed.
Journal: Journal of Pharmaceutical Analysis - Volume 3, Issue 3, June 2013, Pages 168–172