کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2553456 1124906 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The concentration-dependent chemokine release and pro-apoptotic effects of neutrophil-derived α-defensin-1 on human bronchial and alveolar epithelial cells
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
The concentration-dependent chemokine release and pro-apoptotic effects of neutrophil-derived α-defensin-1 on human bronchial and alveolar epithelial cells
چکیده انگلیسی

Defensins play a pivotal role in antimicrobial reactions, inflammatory responses, wound repair, and specific immunity. In inflammatory and infectious lung diseases, α-defensins are released from recruited neutrophils, and modulate a variety of lung cell functions. We found that human bronchial and alveolar epithelial cells treated with low and moderate concentrations (5 and 10 μg/ml) of purified neutrophil-derived α-defensin-1 secreted more interleukin (IL)-8 and monocyte chemoattractant protein (MCP)-1 in a dose- and time-dependent manner. Under moderate and high concentrations (10 and 20 μg/ml) of α-defensin-1, we observed typical apoptotic changes in the lung epithelial cells after stimulation for 24 h. Furthermore, α-defensin-1 triggered lung cell detachment in a time- and dose-dependent manner at moderate and high concentrations. Prior to the detachment, caspase-3 activity significantly increased. On confocal laser microscopy, rapid translocation of α-defensin-1 to the endoplasmic reticulum (ER) was noted. These findings suggest that neutrophil-derived α-defensin-1 has pro-inflammatory and apoptotic effects in human bronchial and alveolar epithelial cells, which are concentration-dependent and may be associated with ER activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences - Volume 80, Issue 8, 30 January 2007, Pages 749–758
نویسندگان
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