کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
25572 43582 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of low frequency knockout mutants in Dictyostelium discoideum created by single or double homologous recombination
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Identification of low frequency knockout mutants in Dictyostelium discoideum created by single or double homologous recombination
چکیده انگلیسی

Generation and characterization of knockout clones is a widely used approach to evaluate the specific function of a gene product in Dictyostelium discoideum. The mutant clones are generally obtained by double homologous recombination in the target gene. A frequent limitation to obtaining mutants is the low frequency of homologous recombination. Here we present an easy method to identify rare mutants, based on PCR analysis of pools of clones. This method also allows the isolation of functional knockout mutants created by a single homologous recombination event, which can be more frequent than a double recombination event.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 122, Issue 1, 9 March 2006, Pages 1–4
نویسندگان
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