کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2562188 | 1127077 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Allyl-, butyl- and phenylethyl-isothiocyanate activate Nrf2 in cultured fibroblasts
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کلمات کلیدی
AITCMAPK/ERK kinase 1γGCSC-Jun NH2 terminal kinaseMEK1PEITCBITCNQO1Heme oxygenase-1JnkFCSCpGERKITCHO-1NAD(P)H:quinone oxidoreductase 1 - NAD (P) H: کینون اکسیدوردوکتاز 1γ-glutamyl cysteine synthetase - γ-گلوتامیل سیتستین سنتتازAllyl isothiocyanate - ایزوتیوسیانات آللییلfetal calf serum - سرم گوساله جنینantioxidant response element - عنصر پاسخ آنتی اکسیدانARE - هستندextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Allyl-, butyl- and phenylethyl-isothiocyanate activate Nrf2 in cultured fibroblasts Allyl-, butyl- and phenylethyl-isothiocyanate activate Nrf2 in cultured fibroblasts](/preview/png/2562188.png)
چکیده انگلیسی
The isothiocyanate sulforaphane (SFN) has been shown to induce phase 2 and antioxidant enzymes in cultured cells and in vivo via a Nrf2 dependent signal transduction pathway. However, little is known regarding the effect of structurally related compounds such as allyl isothiocyanate (AITC), butyl isothiocyanate (BITC) and phenylethyl isothiocyanate (PEITC) on Nrf2 target gene expression. In this study AITC, BITC and PEITC significantly increased phosphorylation of ERK1/2, an upstream target of Nrf2 in NIH3T3 fibroblasts. EKR1/2 phosphorylation was accompanied by an increased nuclear translocation and transactivation of Nrf2. AITC, BITC and PEITC significantly enhanced mRNA and protein levels of the Nrf2 targets γ-glutamyl cysteine synthetase (γGCS), heme oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase (NQO1). HO-1 and γGCS both contain CpG islands within their promoter region. However, analysis of DNA methylation status in NIH3T3 cells indicated that expression of these genes may not be dependant on promoter methylation. Current data indicate that not only SFN but also other aliphatic and aromatic isothiocyanates such as AITC, BITC and PEITC induce phase 2 and antioxidant enzymes in cultured fibroblasts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pharmacological Research - Volume 63, Issue 3, March 2011, Pages 233-240
Journal: Pharmacological Research - Volume 63, Issue 3, March 2011, Pages 233-240
نویسندگان
Insa M.A. Ernst, Anika E. Wagner, Christine Schuemann, Niels Storm, Wolfgang Höppner, Frank Döring, Achim Stocker, Gerald Rimbach,