کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2570233 | 1128575 | 2009 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gel entrapment culture of rat hepatocytes for investigation of tetracycline-induced toxicity
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کلمات کلیدی
PBS5,5-dithiobis(2-nitrobenzoic acid)MDACRATRFUDCIPPARDTNBRT-PCRGAPDHGSHTBACyPRat hepatocytes - hepatocytes موشMTT - MTTROS - ROSAdenosine Triphosphate - آدنوزین تری فسفاتATP - آدنوزین تری فسفات یا ATPSteatohepatitis - استاتو هپاتیتnonalcoholic steatohepatitis - استاتو هپاتیت غیر الکلیThiobarbituric acid - اسید تیوباربیتوریکTetracycline - تتراسایکلینGel entrapment - جذب ژلHepatotoxicity - سمیت کبدcytochrome - سیتوکرومmalondialdehyde - مالون دی آلدهیدmethyl thiazolyl tetrazolium - متیل تیزولیل تترازولیمphosphate buffer solution - محلول بافر فسفاتNash - نوشRelative Fluorescence Units - واحد فلورسانس نسبیreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسcarnitine acyltransferase - کارنیتین آسیل ترانسفرازGlutathione - گلوتاتیونglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This paper aimed to explore three-dimensionally cultured hepatocytes for testing drug-induced nonalcoholic steatohepatitis. Gel entrapped rat hepatocytes were applied for investigation of the tetracycline-induced steatohepatitis, while hepatocyte monolayer was set as a control. The toxic responses of hepatocytes were systematically evaluated by measuring cell viability, liver-specific function, lipid accumulation, oxidative stress, adenosine triphosphate content and mitochondrial membrane potential. The results suggested that gel entrapped hepatocytes showed cell death after 96 h of tetracycline treatment at 25 μM which is equivalent to toxic serum concentration in rats, while hepatocyte monolayer showed cell death at a high dose of 200 μM. The concentration-dependent accumulation of lipid as well as mitochondrial damage were regarded as two early events for tetracycline hepatotoxicity in gel entrapment culture due to their detectability ahead of subsequent increase of oxidative stress and a final cell death. Furthermore, the potent protection of fenofibrate and fructose-1,6-diphosphate were evidenced in only gel entrapment culture with higher expressions on the genes related to β-oxidation than hepatocyte monolayer, suggesting the mediation of lipid metabolism and mitochondrial damage in tetracycline toxicity. Overall, gel entrapped hepatocytes in three-dimension reflected more of the tetracycline toxicity in vivo than hepatocyte monolayer and thus was suggested as a more relevant system for evaluating steatogenic drugs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 238, Issue 2, 15 July 2009, Pages 178-187
Journal: Toxicology and Applied Pharmacology - Volume 238, Issue 2, 15 July 2009, Pages 178-187
نویسندگان
Chong Shen, Qin Meng, Eva Schmelzer, Augustinus Bader,