کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2570489 | 1128588 | 2009 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Down-regulation of the detoxifying enzyme NAD(P)H:quinone oxidoreductase 1 by vanadium in Hepa 1c1c7 cells
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کلمات کلیدی
PMSFNQO1CHXAHRnuclear factor erythroid 2-related factor-2TCDDAct-DNrf2C12Kelch-like ECH associating protein 12,3,7,8-Tetrachlorodibenzo-p-dioxin - 2،3،7،8-تترا کلریدیبنزوپتوفان دیوکسینkeap1 - buy1DMSO - DMSONAD(P)H:quinone oxidoreductase 1 - NAD (P) H: کینون اکسیدوردوکتاز 1Electrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزactinomycin D - اکتینومایسین DSUL - دربارهDimethylsulfoxide - دیمتیل سولفواکسیدEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکcycloheximide - سیکلوهایسیمیدantioxidant responsive element - عنصر پاسخ دهنده آنتی اکسیدانHeavy metals - فلزات سنگینphenylmethylsulphonyl fluoride - فنیل متیل سولفونیل فلورایدARE - هستندVanadium - وانادیومaryl hydrocarbon receptor - گیرنده آرویل هیدروکربن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Recent data suggest that vanadium (V5+) compounds exert protective effects against chemical-induced carcinogenesis, mainly through modifying various xenobiotic metabolizing enzymes. In fact, we have shown that V5+ down-regulates the expression of Cyp1a1 at the transcriptional level through an ATP-dependent mechanism. However, incongruously, there is increasing evidence that V5+ is found in higher amounts in cancer cells and tissues than in normal cells or tissues. Therefore, the current study aims to address the possible effect of this metal on the regulation of expression of an enzyme that helps maintain endogenous antioxidants used to protect tissues/cells from mutagens, carcinogens, and oxidative stress damage, NAD(P)H:quinone oxidoreductase 1 (Nqo1). In an attempt to examine these effects, Hepa 1c1c7 cells and its AhR-deficient version, c12, were treated with increasing concentrations of V5+ in the presence of two distinct Nqo1 inducers, the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and isothiocyanate sulforaphane (SUL). Our results showed that V5+ inhibits the TCDD- and SUL-mediated induction of Nqo1 at mRNA, protein, and catalytic activity levels. At transcriptional level, V5+ was able to decrease the TCDD- and SUL-induced nuclear accumulation of Nrf2 and the subsequent binding to antioxidant responsive element (ARE) without affecting Nrf2 protein levels. Looking at post-transcriptional level; we found that V5+ did not affect Nqo1 mRNA transcripts turn-over rates. However, at the post-translational level V5+ increased Nqo1 protein half-life. In conclusion, the present study demonstrates that V5+ down-regulates Nqo1 at the transcriptional level, possibly through inhibiting the ATP-dependent activation of Nrf2.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 236, Issue 3, 1 May 2009, Pages 261-269
Journal: Toxicology and Applied Pharmacology - Volume 236, Issue 3, 1 May 2009, Pages 261-269
نویسندگان
Anwar Anwar-Mohamed, Ayman O.S. El-Kadi,