کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2580839 | 1561636 | 2013 | 9 صفحه PDF | دانلود رایگان |
Phagocytic cells, especially neutrophils (PMNs) are specialized in the production of reactive oxygen species (ROS) to kill pathogenic agents, but an excessive ROS production is associated with tissue damages and inflammatory diseases. Phagocytes are thus prime therapeutic targets to control inflammatory events associated to ROS production. Nowadays, there is a growing interest for the use of polyphenols to modulate the inflammatory response. The aim of this work was to study the antioxidant effect of NDS27, a highly water-soluble form of the polyphenolic molecule curcumin, on in vitro stimulated equine PMNs and human promyelocytic leukemia cells (HL-60). NDS27 was either pre-incubated with cells and eliminated before their activation (intracellular effect) or let in the medium (extracellular effect). Our results indicate that NDS27 significantly and dose-dependently (10−6 M–10−4 M) inhibited the ROS production in both cell types without affecting their viability. NDS27 was able to cross and interact with cell membrane, especially for HL-60 cells, while we observed a better intracellular antioxidant effect with PMNs. The activity of myeloperoxidase (MPO) released by PMNs and HL-60 cells, was decreased by NDS27, but more efficiently for PMNs. These results suggested that the greater efficiency of NDS27 in PMNs is due to an inhibitory effect on cells which are more mature for ROS production, probably by targeting the enzymes implied in respiratory burst like MPO. The modulatory effect of NDS27 on the oxidant activity of cells involved in immune and inflammatory responses opens perspectives for a therapeutic control of pathologies with excessive inflammatory reactions.
► A new water-soluble form of curcumin called NDS27 was synthesized.
► NDS27 has extra- and intracellular antioxidant effects on stimulated neutrophils and HL-60 cells.
► NDS27 enters into the cells and interacts easily with their membranes.
► The antioxidant effect of NDS27 is more efficient with neutrophils than HL-60 cells.
► The antioxidant activity of NDS27 combines a ROS scavenging effect and an anti-catalytic effect on myeloperoxidase activity.
Journal: Chemico-Biological Interactions - Volume 201, Issues 1–3, 25 January 2013, Pages 49–57