Catabolism of 4-hydroxy-2-trans-nonenal by THP1 monocytes/macrophages and inactivation of carboxylesterases by this lipid electrophile

► Effects of 4-hydroxynonenal (HNE) on esterase and lipase activities was examined. ► Hydrolytic activities of pure CES enzymes and intact cells are inhibited by HNE. ► Lysine 105 in recombinant CES1 enzyme was covalently modified by HNE. ► THP1 cells efficiently metabolize HNE and yield glutathione-HNE conjugates.