Amphetamine-evoked changes of oxidative stress and neuropeptide Y gene expression in hypothalamus: Regulation by the protein kinase C-δ signaling

Amphetamine (AMPH), a psychostimulant, is an appetite suppressant and may be regarded as a neurotoxin. It was reported that superoxide dismutase (SOD) and neuropeptide Y (NPY) participated in AMPH-mediated behavior response. However, molecular mechanisms underlying this action are not well known. Using feeding behavior as an indicator, this study investigated if protein kinase C (PKC)-δ signaling was involved. Rats were given daily with AMPH for 4 days. Changes in hypothalamic NPY, PKCδ and SOD mRNA contents were measured and compared. Results showed that the up-regulations of PKCδ and SOD mRNA levels following AMPH treatment were concomitant with the down-regulation of NPY mRNA level and the decrease of feeding. To further determine if PKCδ was involved, intracerebroventricular infusions of PKCδ antisense oligonucleotide were performed at 1 h before daily AMPH treatment in freely moving rats, and results showed that PKCδ knock-down could block the anorectic response and restore partially both NPY and SOD mRNA levels in AMPH-treated rats. It is suggested that central PKCδ signaling may play a functional role in the regulation of AMPH-mediated appetite suppression via a modification of hypothalamic NPY gene expression. Moreover, the increase of SOD during AMPH treatment may favor this modification.