کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2595249 1132302 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Steroid secretion following exposure of ovarian follicular cells to three different natural mixtures of persistent organic pollutants (POPs)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Steroid secretion following exposure of ovarian follicular cells to three different natural mixtures of persistent organic pollutants (POPs)
چکیده انگلیسی

This study investigated in vitro endocrine disrupting effects of three mixtures of POPs: ‘Marine mix’ extracted from Atlantic cod liver, and two mixtures extracted from burbot liver, ‘Mjøsa mix’ and ‘Losna mix’.The POP mixtures were chemically characterized. Co-culture of theca and granulosa cells, were exposed for 48 h with different doses of ‘Marine mix’, ‘Mjøsa mix’ or ‘Losna mix’. As an end point cell viability was determinated by LDH test, steroid analysis by EIA and caspase-3 by colorimetric substrate.Chemical characterization of the mixtures demonstrated that the ‘Marine mix’ contained high levels of DDTs and PCBs. In the ‘Mjøsa mix’, the dominant pollutants were BDEs and HBCD. The concentrations of POPs measured in the ‘Losna mix’ were considerably lower.All mixtures used in the present study had a stimulatory effect on testosterone and estradiol secretion with ‘Marine mix’ > ‘Mjøsa mix’ > ‘Losna mix’. These results show that even a mixture containing background concentrations of POPs significantly affected steroid secretion. A higher steroidogenic response in low dose ranges, compared with high dose ranges indicated xenobiotic-conditioning hormesis. This could complicate predictions of effects in risk assessments.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Toxicology - Volume 25, Issue 1, January 2008, Pages 58–66
نویسندگان
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