کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2602478 1133762 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Diosmin induces genotoxicity and apoptosis in DU145 prostate cancer cell line
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Diosmin induces genotoxicity and apoptosis in DU145 prostate cancer cell line
چکیده انگلیسی


• Flavonoids lowered cancer cell viability and proliferation in a different manner.
• Diosmin provoked apoptosis in DU145 cells.
• Flavonoids induced oxidative stress.
• Diosmin was found a potent inducer of DSBs and chromosomal damage.
• Diosmin-induced genotoxicity may result in cytotoxicity against cancer cells.

Plant-derived dietary polyphenolic compounds, such as flavonoids, with cancer cell-specific pro-apoptotic activity and chemopreventive potential are thought to be promising anticancer agents. In the present study, we were interested in determining if flavonoid-induced genotoxicity may also provoke cancer cell death. Cyto- and genotoxicity of three selected flavonoid glycosides (naringin, diosmin and hesperidin) in DU145 prostate cancer cell line were investigated. Flavonoid glycosides decreased cancer cell number and proliferative activity in a different manner. Flavonoid glycosides induced oxidative stress: intracellular total ROS and superoxide production were augmented after flavonoid treatment. Flavonoid glycosides stimulated DNA double strand breaks (DSBs) and micronuclei production. Diosmin was found the most potent genotoxic agent in DU145 cells, which, in turn, resulted in its pro-apoptotic activity. The more robust recruitment of 53BP1 was correlated with lower DNA and chromosomal damage after naringin and hesperidin treatment compared with diosmin treatment. Flavonoid glycosides were also found to be DNA hypomethylating agents with an ability to modulate cancer cell epigenome leading to changes in the gene expression patterns. Taken together, diosmin, a dietary flavonoid glycoside, was found active against DU145 cells by promoting genotoxic events and a concomitant apoptotic cell death. Thus, a comprehensive analysis of biological activity of diosmin against cancer cells both in vitro and in vivo deserves further investigation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 29, Issue 3, April 2015, Pages 417–425
نویسندگان
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