کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2602960 | 1133802 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro determination of tetanus toxicity by an endopeptidase assay linked to a ganglioside-binding step
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کلمات کلیدی
PBSH-chainL-chainGangliosidesBoNTTeNTTMB1,4-piperazinediethanesulfonic acid - 1،4-piperazinediethanesulfonic acid3,3′,5,5′-tetramethylbenzidine - 3،3 '، 5،5'-تترامیلیل بنزیدینBSA - BSAbovine serum albumin - آلبومین سرم گاوtetanus neurotoxin - ترومبوس نوروتوکسینBinding assay - تست اتصالIn vitro - درونکشتگاهیlight chain - زنجیره سبکheavy chain - زنجیره سنگینTCEP - ساکتSynaptobrevin - سیناپتوبرینEuropean Pharmacopoeia - فارماکوپه اروپاییPhosphate buffered saline - فسفات بافر شورPipes - لوله هایBotulinum neurotoxin - نوروتوکسین بوتولینومClostridial neurotoxins - نوروتوکسین کلستریاید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Assays for the detection of tetanus neurotoxin (TeNT) are relevant for research applications as well as for the safety testing of tetanus vaccines. So far, these assays are usually performed as toxicity tests in guinea pigs or mice. The alternative methods described to date were mostly based on the detection of the toxin's proteolytic activity. However, these endopeptidase assays turned out to be unreliable because they only measure the enzymatic activity as sole determinant of tetanus toxicity, while not taking into account other parameters like the toxin's capacity to bind to target cells. In order to better reflect the in vivo situation of a tetanus infection, we have linked an endopeptidase assay to a ganglioside-binding step. The resulting method, which offers a unique combination of two functionally linked assays, detects those TeNT molecules only which possess both a functional binding domain as well as an active enzymatic domain. Our results demonstrate that this assay is able to reliably detect TeNT, and therefore might provide a basis for the replacement of the animal tests for detection of tetanus toxicity. Moreover, the assay concept could also be useful for in vitro toxicity measurements of other toxins with similar subunit structures.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 24, Issue 3, April 2010, Pages 988-994
Journal: Toxicology in Vitro - Volume 24, Issue 3, April 2010, Pages 988-994
نویسندگان
Heike A. Behrensdorf-Nicol, Ursula Bonifas, Birgit Kegel, Katja Silberbach, Beate Krämer, Karin WeiÃer,