کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2602982 1133803 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Paeoniflorin protects RAW 264.7 macrophages from LPS-induced cytotoxicity and genotoxicity
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Paeoniflorin protects RAW 264.7 macrophages from LPS-induced cytotoxicity and genotoxicity
چکیده انگلیسی

LPS is one of the major constituents of the outer membrane of Gram-negative bacteria. LPS-induced activation of macrophage results in the nitrite (NO) production and the secretion of a pro-inflammatory mediator such as PGE2. Excessive NO reacts with the superoxide anion to generate a selective oxidant and nitrating agent, peroxynitrite, which interacts with biological molecules and damages the cell membranes of them, being able to result in the cell death. We evaluated the protective effects of paeoniflorin (PF) against LPS-induced toxicity by measuring its dose-dependent effects on cell viability in MTT assay, NO production in NO assay, PGE2 production in prostaglandin E2 (PGE2) assay, and DNA damage in comet assay in LPS-treated RAW 264.7 macrophages. In the comet assay, we also analyzed tail length, tail DNA, and tail moment as the markers of DNA strand breaks. PF-treatment significantly protected RAW 264.7 macrophages against LPS-induced toxicity with the increase of viable cells, the decrease of NO and PGE2, and the repair of DNA damage.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 23, Issue 6, September 2009, Pages 1014–1019
نویسندگان
, ,