کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2602989 | 1133803 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bax-associated mechanisms underlying the response of embryonic cells to methotrexate
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
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چکیده انگلیسی
Bax was shown previously to regulate apoptotic cell death in various experimental systems, however, its involvement in teratogen-induced apoptosis is not clear yet. Therefore, we explored the involvement of Bax in the response of mouse embryonic fibroblasts (MEFs) to the anti cancer drug methotrexate (MTX), using Bax wild type (WT) and knockout (Baxâ/â) MEFs. Our results demonstrated a significant teratogen-induced dose- and time-dependant decrease in the survival and culture density of both cell lines, which were found to be somewhat more prominent in WT cells. Exposure to MTX resulted also in decreased cell proliferation of WT but not Baxâ/â cells and accordingly, we observed an accumulation of cells in the S phase and an increased percentage of cells in the Sub-G1 phase of the cell cycle and the appearance of condensed nuclei, which were found to be somewhat more prominent in WT MEFs. In parallel, WT MEFs demonstrated a MTX-induced increase in the percentage of Bax-positive cells and a significant decrease in the percentage of bcl-2-, p65- or IκBα-positive cells, which were not detected in Baxâ/â MEFs. Altogether, the differential sensitivity of WT or Baxâ/â MEFs to MTX suggests a possible involvement of this molecule in the response of embryonic cells to teratogens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 23, Issue 6, September 2009, Pages 1062-1068
Journal: Toxicology in Vitro - Volume 23, Issue 6, September 2009, Pages 1062-1068
نویسندگان
S. Savion, E. Shtelman, H. Orenstein, A. Torchinsky, A. Fein, V. Toder,