کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2788062 | 1154379 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of the Î12 fatty acid desaturase gene between high-oleic and normal-oleic peanut genotypes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
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![عکس صفحه اول مقاله: Comparison of the Î12 fatty acid desaturase gene between high-oleic and normal-oleic peanut genotypes Comparison of the Î12 fatty acid desaturase gene between high-oleic and normal-oleic peanut genotypes](/preview/png/2788062.png)
چکیده انگلیسی
Î12 fatty acid desaturase gene has been targeted as a logical candidate controlling the high oleate trait in peanut seeds. By RT-PCR method, the full-length cDNAs of Î12 fatty acid desaturase gene were isolated from peanut (Arachis hypogaea L.) genotypes with normal and high ratio of oleic to linoleic acid, which were designated AhFAD2B and AhFAD2Bâ², respectively. Sequence alignment of their coding regions revealed that an extra A was inserted at the position +442 bp of AhFAD2Bâ² sequence of high oleic acid genotypes, which resulted in the shift of open reading frame and a truncated protein AhFAD2Bâ², with the loss of one histidine box involved in metal ion complex required for the reduction of oxygen. Analysis of transcript level showed that the expression of Î12 fatty acid desaturase gene in high oleic acid genotype was slightly lower than that in normal genotype. The enzyme activity experiment of yeast (Saccharomyces cerevisiae) cell transformed with AhFAD2B or AhFAD2Bâ² proved that only AhFAD2B gene product showed significant Î12 fatty acid desaturase activity, but AhFAD2Bâ² gene product did not. These results suggested that the change of AhFAD2Bâ² gene sequence resulted in lower activity or deactivation of Î12 fatty acid desaturase in high oleic acid genotype.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Genetics and Genomics - Volume 35, Issue 11, November 2008, Pages 679-685
Journal: Journal of Genetics and Genomics - Volume 35, Issue 11, November 2008, Pages 679-685
نویسندگان
Shanlin Yu, Lijuan Pan, Qingli Yang, Ping Min, Zengkai Ren, Hongsheng Zhang,