کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2811345 | 1569143 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Branchial transcriptional responses of spotted scat, Scatophagus argus, to acute hypo-osmotic stress
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کلمات کلیدی
Transgelin 2SGK1SAMHD1TfR2Aquaporin-3HSP47PERPMRCSArf1IQGAP1MyoferlinS100A13TAGLN2SAM domain and HD domain-containing protein 1phosphate-buffered saline containing Triton X-100Fat2ADP-ribosylation factor 1AQP3PBSTSSHhptMS-222DEPCCXCR4DPTESTs - EST هاFresh water - آب تازهSalt water - آب نمکcycle threshold - آستانه چرخهOsmotic stress - استرس اسمزیScatophagus argus - اسکات فوگاس آرگوسGene expression - بیان ژنtricaine methanesulfonate - تری کینین متانسولفوناتExpressed sequence tags - تگ های توالی بیان شدهdiethyl pyrocarbonate - دیورتیک پریکو کربناتPillar cell - سلول ستونMitochondrion-rich cells - سلول های غنی از میتوکندریونCytochrome oxidase subunit I - سیتوکروم اکسیداز زیر واحد IC-X-C chemokine receptor type 4 - نوع گیرنده شیمیایی C-X-C 4Gene ontology - هستیشناسی ژنیSuppression subtractive hybridization - هیبریداسیون تفکیک پذیری سرکوبHeat shock protein 47 - پروتئین شوک حرارت 47Cox I - کاکس من
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Branchial transcriptional responses of spotted scat, Scatophagus argus, to acute hypo-osmotic stress Branchial transcriptional responses of spotted scat, Scatophagus argus, to acute hypo-osmotic stress](/preview/png/2811345.png)
چکیده انگلیسی
The spotted scat (Scatophagus argus) is a euryhaline marine fish with the capability to withstand different salinity levels and can tolerate abrupt transfer from salt water (SW) to fresh water (FW) or vice versa. Differentially expressed genes were identified by constructing suppression subtractive hybridization (SSH) cDNA libraries to explore branchial osmoregulatory mechanisms affected by salinity challenge. After trimming and blasting, 105 uniquely expressed sequence tags were identified. Among them, 24 candidate genes involved in the stress response, metabolism, and the respiratory chain were chosen for further validation by real-time polymerase chain reaction. More than two-fold expression differences were observed in ADP-ribosylation factor 1 (arf1), cyclic AMP-responsive element-binding protein 3-like 4 (creb3l4), EVI5-like protein (evi5l), protocadherin fat 2 (fat2), transferrin receptor protein 2 (tfr2), C-X-C chemokine receptor type 4 (cxcr4), aquaporin-3 (aqp3), sodium/potassium-transporting ATPase subunit beta-233 (nka β233), serum and glucocorticoid-regulated kinase 1 (sgk1), and ras GTPase-activating-like protein IQGAP1 (iqgap1). Among these genes, aqp and nka are important osmoregulatory factors. AQP3 protein was observed being localized to the membranes of mitochondrion-rich cells (MRCs) and pillar cells of the gill of S. argus, and NKA β233 subunit isoform was only found in MRC membranes by immunostaining. Significant differences in aqp3 and nka β233 expression occurred within 24 h after being transferring into the freshwater, and nka β233 expression level continued to highly upregulated 2 and 7 days post-transfer (dpt). These results suggest that branchial aqp3 and nka β233 play important roles in response to hypo-osmotic stress in S. argus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Agri Gene - Volume 1, August 2016, Pages 100-108
Journal: Agri Gene - Volume 1, August 2016, Pages 100-108
نویسندگان
Maoliang Su, Pan Hu, Jianan Zhou, Jie Ma, Siqi Li, Lang Gui, Junbin Zhang,