کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2820825 | 1570102 | 2013 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Transcriptome dynamics during human erythroid differentiation and development Transcriptome dynamics during human erythroid differentiation and development](/preview/png/2820825.png)
• We constructed a model to study the erythroid development in different stages.
• Distinct gene expression patterns were found during erythroid development.
• We identified dynamic networks and central nodes in stage transitions.
• We identified pathways that may play important roles in different stages.
To explore the mechanisms controlling erythroid differentiation and development, we analyzed the genome-wide transcription dynamics occurring during the differentiation of human embryonic stem cells (HESCs) into the erythroid lineage and development of embryonic to adult erythropoiesis using high throughput sequencing technology. HESCs and erythroid cells at three developmental stages: ESER (embryonic), FLER (fetal), and PBER (adult) were analyzed. Our findings revealed that the number of expressed genes decreased during differentiation, whereas the total expression intensity increased. At each of the three transitions (HESCs–ESERs, ESERs–FLERs, and FLERs–PBERs), many differentially expressed genes were observed, which were involved in maintaining pluripotency, early erythroid specification, rapid cell growth, and cell–cell adhesion and interaction. We also discovered dynamic networks and their central nodes in each transition. Our study provides a fundamental basis for further investigation of erythroid differentiation and development, and has implications in using ESERs for transfusion product in clinical settings.
Journal: Genomics - Volume 102, Issues 5–6, November–December 2013, Pages 431–441