کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2844 137 2015 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Metabolomic profiling coupled with metabolic network reveals differences in Gluconacetobacter xylinus from static and agitated cultures
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Metabolomic profiling coupled with metabolic network reveals differences in Gluconacetobacter xylinus from static and agitated cultures
چکیده انگلیسی


• Both static and agitated cultures have their own disadvantages on producing BC.
• The metabolomic profile of G. xylinus was investigated by GC/MS based metabolomics.
• At high rotational speeds, glucose was converted to gluconic acid rather than BC.
• Accumulation of trehalose in agitated culture facilitated branched pathway.

Both static and agitated culture methods have their own disadvantages on producing bacterial cellulose (BC). To reveal the metabolic differences between these two culture methods, the intracellular metabolic profile characterization of Gluconacetobacter xylinus was investigated using gas chromatography coupled with mass spectrometry. A total of 79 intracellular metabolites in G. xylinus were detected and quantified. Trehalose was mainly responsible for the discrimination among different cultural groups. Coupled with metabolic network, BC production was divided into two stages in agitated culture. At the first stage, glucose was more converted to gluconic acid at high rotational speeds, resulting in a low conversion rate of glucose to BC. At the second stage, gluconic acid acted as carbon source pool for BC synthesis under glucose-limited conditions. However, the accumulation of self-protection metabolites (trehalose, amino acids and gluconic acid) on the 2nd day at 280 rpm branches much carbon source from BC synthesis, resulting in the inhibition of BC synthesis. The highest conversion rate of glucose to BC was observed in static culture, attributing to the inhibition of Glucose-6-phosphate dehydrogenase and the accumulation of by-products. These might be the possible reasons that G. xylinus in static and agitated cultures gave distinct BC yields.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 101, 15 September 2015, Pages 85–98
نویسندگان
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