کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
29431 44397 2014 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthesis, crystal structures, in vitro DNA binding, antibacterial and cytotoxic activities of new di- and polynuclear silver(I) saccharinate complexes with tertiary monophosphanes
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Synthesis, crystal structures, in vitro DNA binding, antibacterial and cytotoxic activities of new di- and polynuclear silver(I) saccharinate complexes with tertiary monophosphanes
چکیده انگلیسی


• New silver(I) saccharinate complexes with tertiary monophosphanes were synthesized and structurally characterized.
• Complexes bind to DNA via intercalation.
• Complexes exhibit high cytotoxic activity against various cancer cells.
• Complexes show remarkable antibacterial activity against various bacteria.

Four new silver(I) saccharinate (sac) complexes, [Ag(μ-sac)(PPh3)]2 (1), [Ag(μ-sac)(PPh2Cy)]2 (2), [Ag(μ-sac)(PPhCy2)]2 (3) and [Ag(μ-sac)(PCy3)]n (4), where PPh3 = triphenylphosphane, PPh2Cy = cyclohexyldiphenylphosphane, PPhCy2 = dicyclohexylphenylphosphane and PCy3 = tricyclohexylphosphane, have been synthesized and fully characterized by elemental analysis, IR, NMR, ESI-MS and single crystal X-ray diffraction. Fluorescence ethidium bromide displacement indicate that all complexes bind to fish sperm (FS) DNA by intercalation with binding constants (KA) of 29.1 ± 0.26 × 105 M−1 for 1, 2.54 ± 0.12 × 105 M−1 for 2, 2.42 ± 0.08 × 105 M−1 for 3, 0.19 ± 0.03 × 105 M−1 for 4. The relative viscosities of the FS-DNA solutions increase with increasing of the complex concentration, providing strong evidence for the intercalation mode. The gel electrophoresis assay further confirms their binding with the pBR322 plasmid DNA. The MIC values of the silver(I) complexes are generally higher than those of AgNO3 and silver sulfadiazine, but 1 presents a promising activity against Salmonellatyphimurium and Staphylococcusaureus. All complexes are highly cytotoxic on human lung carcinoma (A549) and human breast adenocarcinoma (MCF-7) cell lines with IC50 values ranging from 0.82 to 3.13 μM.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 131, 5 February 2014, Pages 31–42
نویسندگان
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