کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
29743 | 44434 | 2011 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effects of blue light irradiation on human dermal fibroblasts Effects of blue light irradiation on human dermal fibroblasts](/preview/png/29743.png)
Previous studies have reported that separately from UV-radiation also blue light influences cellular physiology in different cell types. However, little is known about the blue light action spectrum. The purpose of this study was to investigate effects of blue light at distinct wavelengths (410, 420, 453, 480 nm) emitted by well defined light-emitting-diodes on viability, proliferation and antioxidative capacity of human dermal fibroblasts. We found that irradiation with blue light (410, 420 nm) led to intracellular oxidative stress and toxic effects in a dose and wavelength dependent manner. No toxicity was observed using light at 453 nm and 480 nm. Furthermore, blue light (410, 420, 453 nm) at low doses reduced the antioxidative capacity of fibroblasts. At non-toxic doses, irradiations at 410, 420 and 453 nm reduced proliferation indicating a higher susceptibility of proliferating fibroblasts to blue light. Our results show that blue light at different wavelengths may induce varying degrees of intracellular oxidative stress with different physiological outcome, which could contribute to premature skin photoaging. On the other hand, the use of blue light due to its antiproliferative and toxic properties may represent a new approach in treatment and prevention of keloids, hypertrophic scars and fibrotic skin diseases.
► We have investigated the effects of blue light on human dermal fibroblasts.
► We used novel Light emitting diodes (LED) emitting at 410,420, 453 and 480 nm.
► Blue light induced wavelength and dose dependent toxicity and oxidative stress.
► Longer wavelengths (453, 480 nm) did not show toxicity but effects on proliferation.
► Possible therapeutical use in the treatment of hypertrophic scars and keloids.
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 103, Issue 2, 3 May 2011, Pages 118–125