کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
29760 44436 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Assessing the mechanism of DNA damage induced by lead through direct and indirect interactions
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Assessing the mechanism of DNA damage induced by lead through direct and indirect interactions
چکیده انگلیسی


• Direct and indirect interactions between lead and DNA were discussed.
• Lead induced a dose-dependent increase in the frequency of DNA strand breaks.
• Pb2+ could bind to DNA by minor groove binding effects and electrostatic forces.
• Suggestions on the DNA damage induced by heavy metal pollutants were provided.

Lead still possesses great threats to human health owing to its widespread distribution in the environment caused by human activities, although various actions have been taken to cut down the use and distribution of lead. In this work, mechanisms of DNA damage caused by lead through indirect and direct interactions were investigated. Results from comet assay showed lead at 1–10 μM induced DNA strand breaks in mice liver cells according to olive tail moment analysis. Signals of DNA–protein crosslinks (DPC) were not significantly detected until exposed at 100 μM Pb2+. Further more, direct interactions between Pb2+ and DNA were explored to determine the binding mode between them using spectra analysis, isothermal titration calorimetry studies and molecular docking investigations, which indicated that Pb2+ could bind to DNA with four binding sites to form Pb2+–DNA complex by minor groove binding effects and electrostatic forces, resulting in damage to the structure of DNA double helix. Combined studies of lead genotoxicity in indirect (comet assay and DPC assay) and direct (binding mode investigations) interactions can be applied to study the potential damages to DNA induced by heavy metal pollutants.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 136, 5 July 2014, Pages 46–53
نویسندگان
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