کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
30108 44460 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Photodynamic inactivation of virulence factors of Candida strains isolated from patients with denture stomatitis
ترجمه فارسی عنوان
غربالگری فوتودینامیک عوامل سرطانی سوند های کاندیدا جدا شده از بیماران دچار استوماتیت دندانپزشکی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• PDI was proposed for the treatment of DS and reduction of Candida pathogenicity.
• Absorption spectrum of ER coincident with LED emission is essential for PDI success.
• Planktonic cultures were more sensitive to PDI than biofilms.
• Biofilms exposed to PDI showed a decrease in fungal structures.
• PDI promoted slight reductions in most of the virulence factors of C. albicans and some of the C. tropicalis strains.

Candida species are major microorganisms isolated in denture stomatitis (DS), an inflammatory process of the mucosa underlying removable dental prostheses, and express a variety of virulence factors that can increase their pathogenicity. The potential of Photodynamic inactivation (PDI) in planktonic culture, biofilms and virulence factors of Candida strains was evaluated. A total of 48 clinical Candida isolates from individuals wearing removable maxillary prostheses with DS were included in the study. The effects of erythrosine (ER, 200 μM) and a green LED (λ 532 ± 10 nm, 237 mW/cm2 and 42.63 J/cm2) in a planktonic culture were evaluated. The effect of the addition of ER at a concentration of 400 μM together with a green LED was evaluated in biofilms. The virulence factors of all of the Candida strains were evaluated before and after the PDI process in cells derived from biofilm and planktonic assays. All of the Candida species were susceptible to ER and green LED. However, the biofilm structures were more resistant to PDI than the planktonic cultures. PDI also promoted slight reductions in most of the virulence factors of C. albicans and some of the Candida tropicalis strains. These results suggest that the addition of PDI is effective for reducing yeasts and may also reduce the virulence of certain Candida species and decrease their pathogenicity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 153, December 2015, Pages 82–89
نویسندگان
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