کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
30280 | 44468 | 2014 | 7 صفحه PDF | دانلود رایگان |
• The interaction antitumor drug, idarubicin, and DNA is investigated.
• Several spectroscopic techniques are used in the study of interaction.
• Fluorescence quenching mechanism of idarubicin and DNA is combined static and dynamic.
• The interaction between idarubicin and DNA is intercalation mode.
The interaction between idarubicin and double stranded deoxyribonucleic acid (ds-DNA) was investigated by UV–VIS spectrophotometry, fluorescence and Raman spectroscopy techniques. The absorption spectra of idarubicin with ds-DNA showed a slight red shift and hypochromic effect. In the fluorescence experiments, emission peaks were decreased by adding ds-DNA. Using ethidium bromide (ETB) as a fluorescence probe, fluorescence quenching of the emission peak was observed in the ETB-DNA system when idarubicin was added. Moreover, similar results were obtained in Raman spectroscopy. Binding constants of idarubicin with ds-DNA were determined as 5.14 × 105 M−1 and 5.8 × 105 M−1 for UV–VIS spectrophotometry and fluorescence spectroscopy, respectively. The large binding constant indicated that idarubicin has a high affinity with ds-DNA. All the evidences indicated that the binding mode of idarubicin with DNA was an intercalative binding. Furthermore, quantitative determination of idarubicin in pharmaceutical formulation was done.
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 138, 5 September 2014, Pages 36–42