کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
30664 | 44495 | 2012 | 8 صفحه PDF | دانلود رایگان |
Fluorescence and absorption spectroscopy, circular dichroism (CD) as well as viscosity experiment have been used to characterize the DNA binding of [Ho(Phen)2Cl3]·H2O, where phen stand for 1,10-phanathroline. This complex exhibits the marked decrease in the emission intensity and some hypochromism in UV–Vis spectrum in the presence of DNA. For characterization of the binding mode between the Ho(III) complex and DNA various procedures such as: absorption and emission titration and EB quenching experiments, viscosity measurements, CD study, iodide quenching assay, salt effect and thermodynamical investigation are used. The intrinsic binding constant of [Ho(Phen)2Cl3]·H2O with DNA is calculated by UV–Vis and florescence spectroscopy. The value of binding constants in 296, 299 and 303 are 1.99 ± 0.07 × 104, 1.07 ± 0.09 × 104 and 0.84 ± 0.06 × 104, respectively. The thermodynamic studies show that the reaction is entropically driven. The above-mentioned physical measurements indicate that the Ho(III) complex binds to fish salmon DNA, presumably via groove binding mode.
In this work, an attempt has been made to investigate the interaction of [Ho(Phen)2Cl3]·H2O with fish salmon DNA using UV–vis, fluorescence spectroscopy, circular dichroism and viscosimetery techniques.Figure optionsDownload as PowerPoint slideHighlights
► Ho(III) complex shows good binding affinity to FS DNA with Kb = 1.07 × 104 M−1.
► Viscosity of DNA almost unchanged by increasing amount of Ho complex.
► CD spectrum of DNA has a little change with increasing amount of Ho complex.
► Thermodynamic parameters indicate that the binding reaction is entropically driven.
► Our studies suggest that the binding mode is groove binding.
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 117, 5 December 2012, Pages 132–139