Effect of UV irradiation on the physico-chemical properties of iron crosslinked collagen

Collagen is the main component of connective tissue and finds immense applications as a biomaterial. In this study, effect of UV irradiation on collagen crosslinked with iron has been carried out. The physical and optical properties of crosslinked collagen affected by UV irradiation were analyzed using electrospectral and fluorescence studies. The electronic spectral studies showed that the photoproducts formed on UV radiation decrease in the presence of iron. Circular dichroic studies revealed that the conformational changes brought about in the protein due to UV irradiation have been reduced owing to the crosslinking with iron. However, prolonged irradiation does bring about conformational changes to the protein.

The effect of UV radiation on iron crosslinked collagen has been investigated. The response of native and iron crosslinked collagen to UV irradiation has been found to differ significantly. Iron crosslinking alleviates the negative effects of UV radiation.Figure optionsDownload as PowerPoint slideHighlights
► Effect of UV radiation on iron crosslinked collagen studied.
► The physical and optical properties were analyzed using electrospectral, fluorescence and conformational studies.
► Iron crosslinking reduces the effect of UV irradiation.