کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
30981 44523 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Photobiological effects of UVA and UVB light in zebrafish embryos: Evidence for a competent photorepair system
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Photobiological effects of UVA and UVB light in zebrafish embryos: Evidence for a competent photorepair system
چکیده انگلیسی

The consequences of UVB and UVA irradiation on hatch rate, mortality, and malformation were studied in embryonic zebrafish (Danio rerio). The use of zebrafish embryos has expanded from traditional developmental models to diverse studies, including many techniques utilizing light exposure. To characterize useful indicators of photodamage, the responses and threshold limits of UV radiation as a function of embryonic stage and fish source were evaluated. Significant differences in UVB susceptibility were observed in embryos at 3, 6–7, 12, and 24 h post-fertilization (hpf), with the 1000-cell stage (3 hpf) having greatest tolerance to UVB. Embryos derived from zebrafish raised in outdoor ponds were more tolerant to UVB than were embryos from laboratory-raised fish. Combinations of UVB and UVA exposure were used to confirm the presence of a competent photorepair system in zebrafish that could return otherwise malformed embryos to a normal phenotype. Overall, embryonic zebrafish had large tolerances (LD50 of 850 J/cm2) to UVA, confirming their suitability for photoactivation and photorepair studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 88, Issues 2–3, 25 September 2007, Pages 137–146
نویسندگان
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