کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3120780 | 1583295 | 2015 | 9 صفحه PDF | دانلود رایگان |
• Intravenously transplanted ADSCs could home to irradiated SGs in 24 h after infusion.
• ADSCs protect SGs against damage induced by high-dose radiation (18 Gy) in mice.
• ADSCs protect both the morphology and function of irradiated SGs.
ObjectiveHigh-dose radiation therapy in the head and neck area can lead to irreversible damage to salivary glands (SGs) with consequent xerostomia. Adipose-derived stem cells (ADSCs) have been shown to repair or rescue damaged SGs. Thus, we investigated the protective efficacy of ADSCs in the prevention of SG damage induced by high dose radiation.MethodsThird-passage ADSCs (1 × 106) were transplanted by intravenous infusion into the tail-vein of 8-week-old C57BL/6 mice, immediately after local irritation at a dose of 18 Gy. The process was repeated twice a week during a period of six consecutive weeks. Eight weeks after radiation, functional evaluations were conducted by measuring salivary flow rate (SFR). Histological, immunohistochemical and transmission electron microscopic (TEM) examinations were performed to analyze microstructural and ultrastructural changes, microvessel density, amylase production, apoptosis, and proliferation activity.ResultsIntravenously administrated ADSCs could home to irradiated SGs within 24 h after infusion, significantly increasing SG weights, improving SFR, and preserving the microscopic morphologies of SGs eight weeks post-radiation. More functional acini, higher amylase production levels, and higher microvessel densities were observed in ADSC-treated SGs than in irradiated SGs. Additionally, enhanced cell proliferation activity and reduced radiation-induced SG apoptosis was observed in the ADSC-treated group when compared with the irradiated group.ConclusionSystemic administration of ADSCs immediately after radiation at a dose of 18 Gy can protect both the morphology and function of SGs eight weeks after radiation in mice, and can be used as a protective measure for the prevention of SG damage induced by high-dose radiation.
Journal: Archives of Oral Biology - Volume 60, Issue 10, October 2015, Pages 1488–1496