کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3120932 1583307 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hyperlipidemia causes changes in inflammatory responses to periodontal pathogen challenge: Implications in acute and chronic infections
ترجمه فارسی عنوان
هیپرلیپیدمی باعث تغییر در پاسخ های التهابی به چالش پاتوژن پریودنتال می شود: پیامدهای در عفونت های حاد و مزمن
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
چکیده انگلیسی


• A weak inflammatory response was observed in HFD rabbits in acute infection.
• HFD rabbits exhibited higher cytokine expression after chronic infections.
• HFD rabbits had a higher level of alveolar bone loss than ND rabbits.
• Monocytes from HFD rabbits and exposed to LPS exhibited reduced level of cytokines.
• Different responses to acute and chronic infections were elicited by HLP.

ObjectiveIn this study, the effect of hyperlipidemia on immune responses to periodontal bacterial infections was investigated.MethodsSixty male New Zealand white rabbits were equally assigned to normal diet (ND) and high-fat diet (HFD) for 6 weeks. Every six rabbits with ND or HFD were orally inoculated with live Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis three times a week for 8 weeks. Also every six rabbits with ND or HFD rabbits were injected intravenously with A. actinomycetemcomitans and P. gingivalis LPS. Periodontal disease severity was quantified by macroscopic and radiographical evaluation. Serum cytokines were examined by enzyme-linked immunosorbent assay. In vitro, peripheral mononuclear cells were collected and stimulated with LPS. Quantitative real-time polymerase chain reaction was used to determine the changes in gene expression of macrophages.ResultsIn the early stages of infection, HFD rabbits were exposed to oral infection and systemic infection developed a weak inflammatory response to the reduced cytokine expression compared with ND rabbits. However, HFD rabbits exhibited higher inflammatory cytokine expression during long-term infections. Moreover, the pronounced changes in inflammatory cytokine expression elicited a significantly increase in bone loss in HFD rabbits with oral infection. Peripheral macrophages harvested from HFD rabbits and exposed to LPS exhibited reduced levels of pro-inflammatory cytokines compared with those from ND rabbits in vitro.ConclusionThese data indicated that hyperlipidemia interfered with immune responses differently. The mechanism is possibly associated with immune paralysis in the acute phase and accumulation of inflammatory mediators in the chronic period.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Oral Biology - Volume 59, Issue 10, October 2014, Pages 1075–1084
نویسندگان
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