کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3205364 | 1587545 | 2013 | 9 صفحه PDF | دانلود رایگان |
BackgroundPsoriasis, lichen planus (LP), and atopic dermatitis (AD) are common chronic inflammatory skin diseases mediated by immune responses.ObjectiveWe used RNA sequencing to investigate messenger RNA expression patterns in peripheral T cells of Chinese patients with psoriasis, LP, or AD and of healthy individuals.MethodsAfter peripheral T-cell proliferation, messenger RNA expression patterns were investigated by RNA sequencing, and 6 randomly selected genes were verified by real-time reverse transcriptase polymerase chain reaction.ResultsSix genes were down-regulated and 33 were up-regulated in these diseases. Gene ontology analysis revealed enrichment of genes involved in positive regulation of T-cell activation. Regulation of nuclear premessenger RNA domain containing 1B (RPRD1B) expression was enhanced in psoriasis.LimitationsThe role of hereditary factors in RPRD1B expression in T cells was not considered. Immunomodulators (thymopeptide, levamisole, BCG polysaccharide, nucleic acid injection, and transfer factor) were previously given to patients with psoriasis and LP, but not to patients with AD; the effects of these immunomodulators on gene expression is uncertain.ConclusionRPRD1B may be involved in T-cell activation in our Chinese psoriatic cohort, and may play a role in stimulating epidermal hyperproliferation.
Journal: Journal of the American Academy of Dermatology - Volume 69, Issue 5, November 2013, Pages e235–e243