Evaluation of angiogenesis in diabetic lower limb wound healing using a natural medicine: A quantitative approach

Increasing incidents of diabetes (mellitus) induced non-healing lower extremity wounds and disease associated amputations have raised significant concerns related to quality of life of afflicted patients. High glucose level in diabetic wounds inhibits the transactivation of angiogenesis related molecules resulting delayed healing progression. Present study investigates the impact of a natural medicine like honey in angiogenesis of non-healing diabetic lower limb wounds. Quantitative assessment of different vessel parameters was performed on in vitro CAM model for validation of angiogenic potential of honey. Further the upregulation of angiogenesis related prime molecular markers like HIF-1α, VEGFA, VEGFR2 is under the therapeutic intervention of honey indicated improved angiogenesis which in turn promote the healing rate. These results may facilitate in determining the healing impact of this natural product in treatment of diabetic wounds and it may also help in developing alternative cost effective therapeutic modality.