کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3392615 | 1221230 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Survival of skin allografts is prolonged in mice with a dominant-negative H-Ras
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کلمات کلیدی
TCrERKRASTregsMAPK - MAPKinterleukin - اینترلوکینTransgenic - تراریختهRegulatory T cells - سلولهای تی تنظیمکنندهdominant negative - غالب منفی استMHC - مجموعه سازگاری بافتی اصلیTransgenic mouse - موش ترانسژنیکwild type - نوع وحشیmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenSkin graft - پیوند پوست، گرافت پوستیT-cell receptor - گیرنده لنفوسیت T
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Ras is a guanine nucleotide-binding protein that plays a major role in regulating the proliferation of T cells. To investigate the mechanism of the Ras/mitogen-activated protein kinase pathway, one of the downstream signal-transduction pathways of T-cell receptors, in the response to alloantigen, we performed full-thickness skin grafting in the major histocompatibility complex (MHC) incompatible strain BALB/c (H-2Kd) (donor) and T-cell-specific H-Ras dominant-negative (dnRas) transgenic (tg) C57BL/6 (H-2Kb) (recipient) male mice. In vitro and in vivo dnRas tg mouse T-cell proliferation and cytotoxic T lymphocyte (CTL) activity assay were also performed. The median graft survival time in control B6/wild type (wt) mouse allografts was seven days. Conversely, the dnRas tg mouse group exhibited a significant (p < 0.01) prolongation of graft survival to 15 days. However, all grafts were eventually rejected after one month. Mixed lymphocyte reaction and popliteal lymph node assay revealed that T-cell proliferation was decreased in response to alloantigen, but CTL activity was not changed in the dnRas tg mice. These results suggested that Ras is essential for peripheral T lymphocytes to respond to allo-MHC antigens, and Ras may be a molecular target for controlling transplant rejection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Transplant Immunology - Volume 18, Issue 4, February 2008, Pages 302-306
Journal: Transplant Immunology - Volume 18, Issue 4, February 2008, Pages 302-306
نویسندگان
Naoko Funeshima-Fuji, Masayuki Fujino, Hiromitsu Kimura, Shiro Takahara, Toshinori Nakayama, Taichi Ezaki, Xiao-Kang Li,