کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3415885 | 1224983 | 2007 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Aberrant life cycle of human immunodeficiency virus type 1 CRF15_01B-like clinical isolates from Thailand in human CD4+ T-cell lines
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کلمات کلیدی
Glyceraldehyde-3-phosphate dehydrogenase genemAbG3PDHPBMCECCCRF01_AECRFPMA - LDC هاMonoclonal antibody - آنتی بادی مونوکلونالReverse transcriptase - ترانس کریپتاز معکوس یا وارونویسELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاSubtype - زیرمجموعهperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیcirculating recombinant form - فرم تجدید پذیرphorbol myristate acetate - فروبل مریستات استاتpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازHIV-1 - ویروس اچ آی وی نوع یکHuman immunodeficiency virus type 1 - ویروس نقص ایمنی بدن نوع 1Life cycle - چرخه زندگی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Human immunodeficiency virus type 1 (HIV-1) is separated into several subtypes and circulating recombinant forms (CRFs). Here, infections of 4 clinical isolates (0-47-1, CU98-26, CU98-28, and CU98-31) from Thailand were examined in human CD4+ T-cell lines, MT-4 and MOLT-4. The CU98-26 isolates in both cells and 0-47-1 in MT-4 established chronic infections, as in control 2 subtype B isolates from Japan, while 0-47-1 in MOLT-4 caused a latent infection. In contrast, CU98-28 and CU98-31 established aberrant infections in both cells. Integrated provirus was detected in all the chronic infections, including 0-47-1 in both cells. In contrast, extrachromosomal circular forms of HIV-1 DNA were detected in CU98-28- and CU98-31-infected cells, whereas the amount of the integrated form was below the limit of detection. Interestingly, phylogenetic trees and sequencing revealed that all the Thai isolates, except 0-47-1, displayed CRF15_01B-like mosaic structures of CRF01_AE with subtype B-like sequences in several regions that were apparently different from those of the inocula in peripheral blood mononuclear cells. Thus, in the infections of most of the above Thai isolates it was suggested that a minor population with mosaic patterns having multiple breakpoints between CRF01_AE and subtype B in the inocula could be selected by the T-cell lines.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbes and Infection - Volume 9, Issue 4, April 2007, Pages 449-459
Journal: Microbes and Infection - Volume 9, Issue 4, April 2007, Pages 449-459
نویسندگان
Jiranan Warachit, Yukie Iwabu, Yong-Gang Li, Gui-Mei Li, Panasda Isarangkura, Madiha S. Ibrahim, Kruavon Balachandra, Shoutaro Tsuji, Kazuyoshi Ikuta,