کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3415951 | 1224985 | 2006 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Two clones obtained from Urabe AM9 mumps virus vaccine differ in their replicative efficiency in neuroblastoma cells Two clones obtained from Urabe AM9 mumps virus vaccine differ in their replicative efficiency in neuroblastoma cells](/preview/png/3415951.png)
A high rate of post-vaccinal aseptic meningitis for Urabe AM9 mumps virus strain is well documented. This strain is composed of two virus variants differing at the nt 1081 (A/G) region in the hemagglutinin-neuraminidase (HN) gene. An association of HN-A1081 variant with neurovirulence has been proposed. In order to test for neurotropism we isolated the HN-A1081 and HN-G1081 virus variants from Urabe AM9 mumps virus vaccine. Sequential passages were performed in monkey kidney Vero cells and human neuroblastoma SH-SY5Y cells. Viral replication was determined by conventional and real-time RT-PCR. The results show that clone HN-A1081 can replicate efficiently in both cell types. However, a defective replication of clone HN-G1081, lacking its genetic marker, was observed after the third passage in neuroblastoma cells. Kinetics assays showed that clone HN-A1081 replicates faster than clone HN-G1081. Viral clones were also inoculated into the brains of newborn rats. Clone HN-A1081 replicated 14 times, while clone HN-G1081 merely duplicated its level over the initial inoculum. These results suggest that there is a selective replication of HN-A1081 mumps virus variants in cells of nervous origin.
Journal: Microbes and Infection - Volume 8, Issue 2, February 2006, Pages 332–339