کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3416041 1224990 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Induction of β-defensin 3 in keratinocytes stimulated by bacterial lipopeptides through toll-like receptor 2
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Induction of β-defensin 3 in keratinocytes stimulated by bacterial lipopeptides through toll-like receptor 2
چکیده انگلیسی

The epidermis, which covers the surface of all mammals, serves as a front line of defense against the invasion of pathogenic microbes and acts as a crucial site for innate immune responses. Various antimicrobial molecules are expressed not only on the surfaces of monocytes but also on epithelial cells. β-Defensins, a family of antimicrobial peptides, are produced by several types of epithelial cells, including keratinocytes. However, the induction pathways for β-defensins in keratinocytes are not fully understood. We hypothesized that bacterial components would trigger the expression of β-defensins in keratinocytes through a toll-like receptor (TLR)–MyD88 signaling pathway that plays important roles in innate immunity. Production of TNF-alpha and IL-1 alpha following stimulation with lipopolysaccharide or bacterial lipopeptides was completely abolished in TLR2&TLR4-doubly deficient keratinocytes and in MyD88-deficient keratinocytes. Expression of murine β-defensin was upregulated by bacterial lipopeptides in wild-type keratinocytes, while it was attenuated in TLR2-deficient keratinocytes. To evaluate the in vivo role of TLRs in keratinocytes, we inoculated Staphylococcus aureus into the tail skin from TLR2-deficient mice that had been grafted on the dorsal skin of syngeneic mice. The grafted skin from TLR2-deficient mice resulted in erosion. These studies strongly suggest that the TLR2–MyD88-dependent pathway in keratinocytes is essential for antimicrobial activity in vivo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbes and Infection - Volume 8, Issue 6, May 2006, Pages 1513–1521
نویسندگان
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