کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3416180 1225007 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular characterization of the rpoB gene in Brucella species: new potential molecular markers for genotyping
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Molecular characterization of the rpoB gene in Brucella species: new potential molecular markers for genotyping
چکیده انگلیسی

The rpoB gene encoding the β subunit of the DNA-dependent RNA polymerase was molecularly characterized by PCR amplification and DNA sequencing in 26 Brucella reference strains by using primers selected according to the B. melitensis 16M rpoB published sequence. Comparison of the rpoB nucleotide sequence of all Brucella strains analysed revealed specific nucleotide variations associated with different Brucella species and biovars. 17 rpoB alleles were recognized and new Brucella typing is proposed. Our results suggest that the rpoB gene polymorphism can be used to identify all Brucella species and most of the biovars, offering an improvement over conventional typing methods.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbes and Infection - Volume 8, Issue 3, March 2006, Pages 860–865
نویسندگان
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