کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3416328 1593696 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Variation among Staphylococcus aureus membrane vesicle proteomes affects cytotoxicity of host cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Variation among Staphylococcus aureus membrane vesicle proteomes affects cytotoxicity of host cells
چکیده انگلیسی


• MVs from clinical Staphylococcus aureus isolates have different cytotoxic activity toward host cells.
• Proteins in S. aureus MVs are directly responsible for the apoptosis of host cells.
• MVs from different S. aureus isolates carry strain-specific proteins.
• S. aureus toxins, Hld, Hlg, leukocidin D, ETA, and ETC, are secreted by MVs.

Staphylococcus aureus secretes membrane-derived vesicles (MVs), which can deliver virulence factors to host cells and induce cytopathology. However, the cytopathology of host cells induced by MVs derived from different S. aureus strains has not yet been characterized. In the present study, the cytotoxic activity of MVs from different S. aureus isolates on host cells was compared and the proteomes of S. aureus MVs were analyzed. The MVs purified from S. aureus M060 isolated from a patient with staphylococcal scalded skin syndrome showed higher cytotoxic activity toward host cells than that shown by MVs from three other clinical S. aureus isolates. S. aureus M060 MVs induced HEp-2 cell apoptosis in a dose-dependent manner, but the cytotoxic activity of MVs was completely abolished by treatment with proteinase K. In a proteomic analysis, the MVs from three S. aureus isolates not only carry 25 common proteins, but also carry ≥60 strain-specific proteins. All S. aureus MVs contained δ-hemolysin (Hld), γ-hemolysin, leukocidin D, and exfoliative toxin C, but exfoliative toxin A (ETA) was specifically identified in S. aureus M060 MVs. ETA was delivered to HEp-2 cells via S. aureus MVs. Both rETA and rHld induced cytotoxicity in HEp-2 cells. In conclusion, MVs from clinical S. aureus isolates differ with respect to cytotoxic activity in host cells, and these differences may result from differences in the MV proteomes. Further proteogenomic analysis or mutagenesis of specific genes is necessary to identify cytotoxic factors in S. aureus MVs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 93, April 2016, Pages 185–193
نویسندگان
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