Use of otolith chemistry to discriminate juvenile Chinook salmon (Oncorhynchus tshawytscha) from different wild populations and hatcheries in Lake Huron

► We collected juvenile Chinook salmon from 17 Lake Huron streams and 7 hatcheries. ► Otolith chemistry (OC) was analyzed using LA-ICP-MS. ► OC differed between rearing environments, geological regions and collection sites. ► OC identified rearing environment, geological region, and collection site of origin. ► OC can be used as a research and management tool in Lake Huron.